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KMID : 1001020140120020077
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Journal of Urologic Oncology
2014 Volume.12 No. 2 p.77 ~ p.82
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Heterogeneous Expressions of Apoptosis-related Genes in Development of Docetaxel-resistance of the PC3DR2 Prostate Cancer Cell Line
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Lee Hak-Min
Ho Jin-Nyoung
Lee Sang-Chul
Oh Jong-Jin
Hong Sung-Kyu
Lee Sang-Eun
Byun Seok-Soo
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Abstract
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Purpose:To understand the role of the apoptotic pathway of docetaxel- resistant human prostate cancer cell line (PC3DR2).
Materials and Methods: PC3DR2 was established from the PC3 prostate cancer cell line by continuous exposure of escalating concentrations of docetaxel. Total RNA of PC3 and PC3DR2 were collected and cDNA microarray test was performed using GeneChipⓡ Human Genome U133 Plus 2.0 Array. GenePlexⓡ software was used for data analysis. Significantly different genes were identified with the cutoff 2.0 of fold change and p-value under 0.05 of Welch’s T-test. The apoptotic pathway heatmap was acquired to identify roles of apoptosis-related genes in development of docetaxel-resistance of PC3DR2.
Results: There were 833 up-regulated and 908 down-regulated genes in the PC3DR2 compared to the control PC3. There were 72 significantly changed KEGG pathways in the PC3DR2 (cutoff: p＜0.05). There were 3 up-regulated (TRADD and 2 FLIPs) and 6 down-regulated (IAP, IKK, Bax, Akt/PKB, PKA and calcineurin) genes in the apoptotic pathway. These 9 changes led to 4 pro-apoptotic and 5 anti-apoptotic processes, indicating contradictory changes in the pathway.
Conclusions: Heterogeneous expressions of apoptosis-related genes were identified in the development of docetaxel resistance of the PC3DR2.
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KEYWORD
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Prostate cancer, Drug resistance, cDNA microarray, Apoptosis
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